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Research & Publications
2010 - 2019
December 25, 2019
A Phase I study of sirolimus in combination withmetronomic therapy (CHOAnome) in children with recurrent or refractory solid and brain tumors
Qayed M, Cash T, Tighiouart M, MacDonald TJ, Goldsmith KC, Tanos R, Kean L, Watkins B, Suessmuth Y, Wetmore C, Katzenstein HM. A Phase I study of sirolimus in combination withmetronomic therapy (CHOAnome) in children with recurrent or refractory solid and brain tumors. Pediatr Blood Cancer. 2020 Apr;67(4):e28134. doi: 10.1002/pbc.28134. Epub 2019 Dec 25.
September 4, 2019
Busulfan combined with immunosuppression allows efficient engraftment of gene-modified cells in a rhesus macaque model
Uchida N, Nassehi T, Drysdale CM, Gamer J, Yapundich M, Bonifacino AC, Krouse AE, Linde N, Hsieh MM, Donahue RE, Dunbar CE, Kean LS, Tisdale JF. Busulfan combined with immunosuppression allows efficient engraftment of gene-modified cells in a rhesus macaque model. Mol Ther. 2019 Sep 4;27(9):1586-1596
May 1, 2019
Characterization of natural killer cells expressing markers associated with maturity and cytotoxicity in children and young adults with sickle cell disease
Abraham AA, Lang H, Meier ER, Nickel RS, Dean M, Lawal N, Speller-Brown B, Wang Y, Kean L, Bollard CM. Characterization of natural killer cells expressing markers associated with maturity and cytotoxicity in children and young adults with sickle cell disease. Pediatric blood & cancer. 2019 May 1; 66(5): e27601. PubMed PMID: 30609269.
October 25, 2018
Evidence for Persistence of the SHIV Reservoir in Multiple Tissues Early After MHC Haplo-identical Hematopoietic Stem Cell Transplantation
Colonna L, Peterson C, Schelle J, Carlson J, Tkachev V, Brown M, Yu A, Reddy S, Obenza W, Nelson V, Polacino P, Mack H, Hu S, Zeleski K, Hoffman M, Olvera J, Furlan S, Zheng H, Taraseviciute A, Hunt D, Betz K, Lane J, Vogel K, Hotchkiss C, Motas C, Baldessari A, Murnane R, English C, Astley C, Wangari S, Agricola B, Athens J, Iwavama N, May A, Stensland L, Huang M, Jerome K, Kiem H, Kean L. Evidence for Persistence of the SHIV Reservoir in Multiple Tissues Early After MHC Haplo-identical Hematopoietic Stem Cell Transplantation. Nature Communications. 2018 Oct 25.
September 27, 2018
Reduced-intensity conditioning for hematopoietic cell transplant for HLH and primary immune deficiencies
Allen CE, Marsh R, Dawson P, Bollard CM, Shenoy S, Roehrs P, Hanna R, Burroughs L, Kean L, Talano JA, Schultz KR, Pai S, Baker KS, Andolina JR, Stenger EO, Connelly J, Ramirez A, Bryant C, Eapen M, Pulsipher MA. Reduced-intensity conditioning for hematopoietic cell transplant for HLH and primary immune deficiencies. Blood. 2018 Sep 27; 132(13): 1438-1451. PubMed PMID: 29997222; PubMed Central PMCID: PMC6161764.
August 31, 2018
CD28 blockade controls T cell activation to prevent graft-versus-host disease in primates. The Journal of clinical investigation
Watkins BK, Tkachev V, Furlan SN, Hunt DJ, Betz K, Yu A, Brown M, Poirier N, Zheng HB, Taraseviciute A, Colonna L, Mary C, Blancho G, Soulillou JP, Panoskaltsis-Mortari A, Sharma P, Garcia A, Strobert E, Hamby K, Garrett A, Deane T, Blazar BR, Vanhove B, Kean LS. CD28 blockade controls T cell activation to prevent graft-versus-host disease in primates. The Journal of clinical investigation. 2018 Aug 31; 128(9): 3991-4007. PubMed PMID: 30102255; PubMed Central PMCID: PMC6118599.
July 24, 2018
The Microbiome and Hematopoietic Cell Transplantation: Past, Present, and Future.
Andermann TM, Peled JU, Ho C, Reddy P, Riches M, Storb R, Teshima T, van den Brink MRM, Alousi A, Balderman S, Chiusolo P, Clark WB, Holler E, Howard A, Kean LS, Koh AY, McCarthy PL, McCarty JM, Mohty M, Nakamura R, Rezvani K, Segal BH, Shaw BE, Shpall EJ, Sung AD, et al. (32 authors). The Microbiome and Hematopoietic Cell Transplantation: Past, Present, and Future. Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation. 2018 Jul 24. PubMed PMID: 29471034.
June 28, 2018
Chimeric Antigen Receptor T Cell-Mediated Neurotoxicity in Non-Human Primates
​Taraseviciute A, Tkachev V, Ponce R, Turtle CJ, Snyder JM, Liggitt HD, Myerson D, Gonzalez-Cuyar L, Baldessari A, English C, Yu A, Zheng H, Furlan SN, Hunt DJ, Hoglund V, Finney O, Brakke H, Blazar BR, Berger C, Riddell S, Gardner R, Kean LS, Jensen MC. Chimeric Antigen Receptor T Cell-Mediated Neurotoxicity in Non-Human Primates. Cancer Discovery. 2018 Jun 28. PubMed PMID: 29563103
April 7, 2018
Human CD19-Targeted Mouse T-cells Induce B-Cell Aplasia and Toxicity in Human CD19 Transgenic Mice
Pennell C, Barnum J, McDonald-Hyman C, Panoskaltsis-Mortari A, Riddle M, Xiong Z, Loschi M, Thangavelu G, Campbell H, Storlie M, Refaeli Y, Furlan S, Jensen M, Kean L, Miller J, Tolar J, Osborn M, Blazar B. Human CD19-Targeted Mouse T-cells Induce B-Cell Aplasia and Toxicity in Human CD19 Transgenic Mice. Molecular Therapy. 2018 Apr 7. Available from: https://www.sciencedirect.com/science/article/pii/S1525001618301631.
January 24, 2018
Building a Safer and Faster CAR: Seatbelts, Airbags, and CRISPR
Perales MA, Kebriaei P, Kean LS, Sadelain M. Building a Safer and Faster CAR: Seatbelts, Airbags, and CRISPR. Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation. 2018 Jan 24; 24(1): 27-31. PubMed PMID: 29032264.
December 1, 2017
In Vitro Induction of Human Regulatory T Cells Using Conditions of Low Tryptophan Plus Kynurenines
Hippen KL, O'Connor RS, Lemire AM, Saha A, Hanse EA, Tennis NC, Merkel SC, Kelekar A, Riley JL, Levine B, June CH, Turka LA, Kean LS, MacMillan ML, Miller JS, Wagner JE, Munn DH, Blazar BR. In Vitro Induction of Human Regulatory T Cells Using Conditions of Low Tryptophan Plus Kynurenines. American journal of Transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 2017 Dec 1; 17(12): 3098-3113. PubMed PMID: 28470889; PubMed Central PMCID: PMC5671378.
September 20, 2017
Combined OX40L and mTOR blockade controls effector T cell activation while preserving Treg reconstitution after transplant
Tkachev V, Furlan SN, Watkins B, Hunt DJ, Zheng HB, Panoskaltsis-Mortari A, Betz K, Brown M, Schell JB, Zeleski K, Yu A, Kirby I, Cooley S, Miller JS, Blazar BR, Casson D, Bland-Ward P, Kean LS. Combined OX40L and mTOR blockade controls effector T cell activation while preserving Treg reconstitution after transplant. Science Translational Medicine. 2017 Sep 20; 9(408). PubMed PMID: 28931653; PubMed Central PMCID: PMC5681253.
March 1, 2017
The Knife's Edge of Tolerance: Inducing Stable Multilineage Mixed Chimerism but With a Significant Risk of CMV Reactivation and Disease in Rhesus Macaques
Zheng HB, Watkins B, Tkachev V, Yu S, Tran D, Furlan S, Zeleski K, Singh K, Hamby K, Hotchkiss C, Lane J, Gumber S, Adams AB, Cendales L, Kirk AD, Kaur A, Blazar BR, Larsen CP, Kean LS. The Knife's Edge of Tolerance: Inducing Stable Multilineage Mixed Chimerism but With a Significant Risk of CMV Reactivation and Disease in Rhesus Macaques. American Journal of Transplantation. 2017 Mar 1; 17(3): 657-670. PubMed PMID: 27500470; PubMed Central PMCID: PMC5338742.
December 1, 2016
Preclinical Testing of Antihuman CD28 Fab' Antibody in a Novel Nonhuman Primate Small Animal Rodent Model of Xenogenic Graft-Versus-Host Disease
Hippen KL, Watkins B, Tkachev V, Lemire AM, Lehnen C, Riddle MJ, Singh K, Panoskaltsis-Mortari A, Vanhove B, Tolar J, Kean LS, Blazar BR. Preclinical Testing of Antihuman CD28 Fab' Antibody in a Novel Nonhuman Primate Small Animal Rodent Model of Xenogenic Graft-Versus-Host Disease. Transplantation. 2016 Dec 1; 100(12): 2630-2639. PubMed PMID: 27861291; PubMed Central PMCID: PMC5175408.
November 24, 2016
Systems analysis uncovers inflammatory Th/Tc17-driven modules during acute GVHD in monkey and human T cells
​Furlan SN, Watkins B, Tkachev V, Cooley S, Panoskaltsis-Mortari A, Betz K, Brown M, Hunt DJ, Schell JB, Zeleski K, Yu A, Giver CR, Waller EK, Miller JS, Blazar BR, Kean LS. Systems analysis uncovers inflammatory Th/Tc17-driven modules during acute GVHD in monkey and human T cells. Blood. 2016 Nov 24; 128(21): 2568-2579. PubMed PMID: 27758873; PubMed Central PMCID: PMC5123196.
November, 2016
Advances in Targeting Co-inhibitory and Co-stimulatory Pathways in Transplantation Settings: The Yin to the Yang of Cancer Immunotherapy
Kean L,Turka L, Blazar B. Advances in Targeting Co-inhibitory and Co-stimulatory Pathways in Transplantation Settings: The Yin to the Yang of Cancer Immunotherapy. Immunol Review. 2016 Nov.
July 1, 2016
Programmed death ligand-1 expression on donor T cells drives graft-versus-host disease lethality
​Saha A, O'Connor RS, Thangavelu G, Lovitch SB, Dandamudi DB, Wilson C, Vincent BG, Tkachev V, Pawlicki JM, Furlan SN, Kean LS, Aoyama K, Taylor P, Panoskaltsis-Mortari A, Foncea R, Ranganathan P, Devine SM, Burrill JS, Guo L, Sacristan C, Snyder NW, Blair IA, Milone MC, Dustin ML, Riley JL, et al. (35 authors). Programmed death ligand-1 expression on donor T cells drives graft-versus-host disease lethality. The Journal of Clinical Investigation. 2016 Jul 1; 126(7): 2642-60. PubMed PMID: 27294527; PubMed Central PMCID: PMC4922691.
December 1, 2015
Immunophenotypic parameters and RBC alloimmunization in children with sickle cell disease on chronic transfusion
Nickel RS, Horan JT, Fasano RM, Meyer E, Josephson CD, Winkler AM, Yee ME, Kean LS, Hendrickson JE. Immunophenotypic parameters and RBC alloimmunization in children with sickle cell disease on chronic transfusion. American Journal of Hematology. 2015 Dec 1; 90(12): 1135-41. PubMed PMID: 26361243.
October 1, 2015
Use of Alefacept for Preconditioning in Multiply Transfused Pediatric Patients with Nonmalignant Diseases
Stenger EO, Chiang KY, Haight A, Qayed M, Kean L, Horan J. Use of Alefacept for Preconditioning in Multiply Transfused Pediatric Patients with Nonmalignant Diseases. Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation. 2015 Oct 1; 21(10): 1845-52. PubMed PMID: 26095669; PubMed Central PMCID: PMC5256634.
July 1, 2015
Red blood cell transfusions are associated with HLA class I but not H-Y alloantibodies in children with sickle cell disease
Nickel RS, Hendrickson JE, Yee MM, Bray RA, Gebel HM, Kean LS, Miklos DB, Horan JT. Red blood cell transfusions are associated with HLA class I but not H-Y alloantibodies in children with sickle cell disease. British Journal of Haematology. 2015 Jul 1; 170(2): 247-56. PubMed PMID: 25891976; PubMed Central PMCID: PMC4490004.
June 18, 2015
CMV reactivation drives posttransplant T-cell reconstitution and results in defects in the underlying TCRβ repertoire
Suessmuth Y, Mukherjee R, Watkins B, Koura DT, Finstermeier K, Desmarais C, Stempora L, Horan JT, Langston A, Qayed M, Khoury HJ, Grizzle A, Cheeseman JA, Conger JA, Robertson J, Garrett A, Kirk AD, Waller EK, Blazar BR, Mehta AK, Robins HS, Kean LS. CMV reactivation drives posttransplant T-cell reconstitution and results in defects in the underlying TCRβ repertoire. Blood. 2015 Jun 18; 125(25): 3835-50. PubMed PMID: 25852054; PubMed Central PMCID: PMC4473113.
May 1, 2015
Immune parameter analysis of children with sickle cell disease on hydroxycarbamide or chronic transfusion therapy
Nickel RS, Osunkwo I, Garrett A, Robertson J, Archer DR, Promislow DE, Horan JT, Hendrickson JE, Kean LS. Immune parameter analysis of children with sickle cell disease on hydroxycarbamide or chronic transfusion therapy. British journal of haematology. 2015 May 1; 169(4): 574-83. PubMed PMID: 25753210; PubMed Central PMCID: PMC4668585.
December 1, 2014
Superiority of rapamycin over tacrolimus in preserving nonhuman primate Treg half-life and phenotype after adoptive transfer
Singh K, Stempora L, Harvey RD, Kirk AD, Larsen CP, Blazar BR, Kean LS. Superiority of rapamycin over tacrolimus in preserving nonhuman primate Treg half-life and phenotype after adoptive transfer. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 2014 Dec 1; 14(12): 2691-703. PubMed PMID: 25359003; PubMed Central PMCID: PMC4236286.
May 1, 2014
Renal transplantation using belatacept without maintenance steroids or calcineurin inhibitors
Kirk AD, Guasch A, Xu H, Cheeseman J, Mead SI, Ghali A, Mehta AK, Wu D, Gebel H, Bray R, Horan J, Kean LS, Larsen CP, Pearson TC. Renal transplantation using belatacept without maintenance steroids or calcineurin inhibitors. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 2014 May 1; 14(5): 1142-51. PubMed PMID: 24684552; PubMed Central PMCID: PMC4642731.
March 20, 2014
CD8-predominant T-cell CNS infiltration accompanies GVHD in primates and is improved with immunoprophylaxis
Kaliyaperumal S, Watkins B, Sharma P, Furlan S, Ramakrishnan S, Giver C, Garcia A, Courtney C, Knight H, Strobert E, Elder E, Crenshaw T, Blazar BR, Waller EK, Westmoreland S, Kean LS. CD8-predominant T-cell CNS infiltration accompanies GVHD in primates and is improved with immunoprophylaxis. Blood. 2014 Mar 20; 123(12): 1967-9. PubMed PMID: 24652969; PubMed Central PMCID: PMC3962171.
November 1, 2013
In vivo T cell costimulation blockade with abatacept for acute graft-versus-host disease prevention: a first-in-disease trial
Koura DT, Horan JT, Langston AA, Qayed M, Mehta A, Khoury HJ, Harvey RD, Suessmuth Y, Couture C, Carr J, Grizzle A, Johnson HR, Cheeseman JA, Conger JA, Robertson J, Stempora L, Johnson BE, Garrett A, Kirk AD, Larsen CP, Waller EK, Kean LS. In vivo T cell costimulation blockade with abatacept for acute graft-versus-host disease prevention: a first-in-disease trial. Biology of blood and marrow transplantation : Journal of the American Society for Blood and Marrow Transplantation. 2013 Nov 1; 19(11): 1638-49. PubMed PMID: 24047754.
2013
Heterologous immunity triggered by a single, latent virus in Mus musculus: combined costimulation- and adhesion- blockade decrease rejection
Beus JM, Hashmi SS, Selvaraj SA, Duan D, Stempora LL, Monday SA, Cheeseman JA, Hamby KM, Speck SH, Larsen CP, Kirk AD, Kean LS. Heterologous immunity triggered by a single, latent virus in Mus musculus: combined costimulation- and adhesion- blockade decrease rejection. PloS one. Forthcoming 2013; 8(8): e71221. PubMed PMID: 23940724; PubMed Central PMCID: PMC3733932.
July 1, 2012
Evidence for kidney rejection after combined bone marrow and renal transplantation despite ongoing whole-blood chimerism in rhesus macaques
​Ramakrishnan SK, Page A, Farris, 3rd AB, Singh K, Leopardi F, Hamby K, Sen S, Polnett A, Deane T, Song M, Stempora L, Strobert E, Kirk AD, Larsen CP, Kean LS. Evidence for kidney rejection after combined bone marrow and renal transplantation despite ongoing whole-blood chimerism in rhesus macaques. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 2012 Jul 1; 12(7): 1755-64. PubMed PMID: 22642491; PubMed Central PMCID: PMC3387328.
June 1, 2012
Regulatory T cells exhibit decreased proliferation but enhanced suppression after pulsing with sirolimus
Singh K, Kozyr N, Stempora L, Kirk AD, Larsen CP, Blazar BR, Kean LS. Regulatory T cells exhibit decreased proliferation but enhanced suppression after pulsing with sirolimus. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 2012 Jun 1; 12(6): 1441-57. PubMed PMID: 22300641; PubMed Central PMCID: PMC3471086.
January 1, 2013
Viewing transplantation immunology through today's lens: new models, new imaging, and new insights
Huang AY, Haining W, Barkauskas DS, Myers JT, Petrosiute A, Garrett AP, Singh K, Cooke KR, Kean LS. Viewing transplantation immunology through today's lens: new models, new imaging, and new insights. Biology of blood and marrow transplantation : Journal of the American Society for Blood and Marrow Transplantation. 2013 Jan 1; 19(1 Suppl): S44-51. PubMed PMID: 23092813; PubMed Central PMCID: PMC4437564.
April 1, 2012
Nonhuman primate transplant models finally evolve: detailed immunogenetic analysis creates new models and strengthens the old. American journal of transplantation
Kean LS, Singh K, Blazar BR, Larsen CP. Nonhuman primate transplant models finally evolve: detailed immunogenetic analysis creates new models and strengthens the old. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 2012 Apr 1; 12(4): 812-9. PubMed PMID: 22177005; PubMed Central PMCID: PMC3482466.
April 1, 2012
Immunogenetic Management Software: a new tool for visualization and analysis of complex immunogenetic datasets
Johnson ZP, Eady RD, Ahmad SF, Agravat S, Morris T, Else J, Lank SM, Wiseman RW, O'Connor DH, Penedo MC, Larsen CP, Kean LS. Immunogenetic Management Software: a new tool for visualization and analysis of complex immunogenetic datasets. Immunogenetics. 2012 Apr 1; 64(4): 329-36. PubMed PMID: 22080300; PubMed Central PMCID: PMC3514553.
February 1, 2012
Alloimmunization to transfused HOD red blood cells is not increased in mice with sickle cell disease
Hendrickson JE, Hod EA, Perry JR, Ghosh S, Chappa P, Adisa O, Kean LS, Ofori-Acquah SF, Archer DR, Spitalnik SL, Zimring JC. Alloimmunization to transfused HOD red blood cells is not increased in mice with sickle cell disease. Transfusion. 2012 Feb 1; 52(2): 231-40. PubMed PMID: 21790627; PubMed Central PMCID: PMC3218203.
January 1, 2012
CD40 blockade combines with CTLA4Ig and sirolimus to produce mixed chimerism in an MHC-defined rhesus macaque transplant model
Page A, Srinivasan S, Singh K, Russell M, Hamby K, Deane T, Sen S, Stempora L, Leopardi F, Price AA, Strobert E, Reimann KA, Kirk AD, Larsen CP, Kean LS. CD40 blockade combines with CTLA4Ig and sirolimus to produce mixed chimerism in an MHC-defined rhesus macaque transplant model. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 2012 Jan 1; 12(1): 115-25. PubMed PMID: 21929643; PubMed Central PMCID: PMC3259212.
December 15, 2011
Significant mobilization of both conventional and regulatory T cells with AMD3100
Kean LS, Sen S, Onabajo O, Singh K, Robertson J, Stempora L, Bonifacino AC, Metzger ME, Promislow DE, Mattapallil JJ, Donahue RE. Significant mobilization of both conventional and regulatory T cells with AMD3100. Blood. 2011 Dec 15; 118(25): 6580-90. PubMed PMID: 21989987; PubMed Central PMCID: PMC3242720.
December 9, 2010
GVHD after haploidentical transplantation: a novel, MHC-defined rhesus macaque model identifies CD28- CD8+ T cells as a reservoir of breakthrough T-cell proliferation during costimulation blockade and sirolimus-based immunosuppression
​Miller WP, Srinivasan S, Panoskaltsis-Mortari A, Singh K, Sen S, Hamby K, Deane T, Stempora L, Beus J, Turner A, Wheeler C, Anderson DC, Sharma P, Garcia A, Strobert E, Elder E, Crocker I, Crenshaw T, Penedo MC, Ward T, Song M, Horan J, Larsen CP, Blazar BR, Kean LS. GVHD after haploidentical transplantation: a novel, MHC-defined rhesus macaque model identifies CD28- CD8+ T cells as a reservoir of breakthrough T-cell proliferation during costimulation blockade and sirolimus-based immunosuppression. Blood. 2010 Dec 9; 116(24): 5403-18. PubMed PMID: 20833977; PubMed Central PMCID: PMC3012549.
November 1, 2010
An MHC-defined primate model reveals significant rejection of bone marrow after mixed chimerism induction despite full MHC matching
Larsen CP, Page A, Linzie KH, Russell M, Deane T, Stempora L, Strobert E, Penedo MC, Ward T, Wiseman R, O'Connor D, Miller W, Sen S, Singh K, Kean LS. An MHC-defined primate model reveals significant rejection of bone marrow after mixed chimerism induction despite full MHC matching. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 2010 Nov 1; 10(11): 2396-409. PubMed PMID: 20849552; PubMed Central PMCID: PMC2980834.
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